5A25A

Rational engineering of a mesophilic carbonic anhydrase to an extreme halotolerant biocatalyst
Slipknot S +31
Knot core range Knot core length Knot tails range Slipknot tails range Slipknot loops range N-end length C-end length Type
view details
+31 25-254 230 1-24, 255-258 24 4 knot
Chain Sequence
HHWGYGKHNGPEHWHKDFPIANGERQSPVDIDTKAVVQDPALKPLALVYGEATSRRMVNNGHSFNVEYDDSQDKAVLKDGPLTGTYRLVQFHFHWGSSDDQGSEHTVDRKKYAAELHLVHWNTKYGDFGTAAQQPDGLAVVGVFLKVGDANPALQKVLDALDSIKTKGKSTDFPNFDPGSLLPNVLDYWTYPGSLTTPPLLESVTWIVLKEPISVSSQQMLKFRTLNFNAEGEPELLMLANWRPAQPLKNRQVRGFPK
Note, that the numbers in the matrix denote the consecutive residues in the loop, not the index of amino acids in the chain!
Knot K 3x +31
Knot core range Knot core length Knot tails range Slipknot tails range Slipknot loops range N-end length C-end length Type
+ 31 24-254 231 1-23, 255-258 23 3 slipknot
Fingerprint Knot forming loop Loop type
K +31
Chain closureHis3 <-> Lys260
... His96 <->
Bridging ionZn280
<-> His94 ... His3
probabilistic
K +31 His3 ... His96 <->
Bridging ionZn280
<-> His119 ...
Chain closureLys260 <-> His3
probabilistic
K +31 His3 ... His94 <->
Bridging ionZn280
<-> His119 ...
Chain closureLys260 <-> His3
probabilistic
Chain Sequence
HHWGYGKHNGPEHWHKDFPIANGERQSPVDIDTKAVVQDPALKPLALVYGEATSRRMVNNGHSFNVEYDDSQDKAVLKDGPLTGTYRLVQFHFHWGSSDDQGSEHTVDRKKYAAELHLVHWNTKYGDFGTAAQQPDGLAVVGVFLKVGDANPALQKVLDALDSIKTKGKSTDFPNFDPGSLLPNVLDYWTYPGSLTTPPLLESVTWIVLKEPISVSSQQMLKFRTLNFNAEGEPELLMLANWRPAQPLKNRQVRGFPK
Whole chain analysis
Subchain analysis 

Knotoid cutoff: 0.5


Knotoid matrix content: 1

Knot core range Knot core length Knot tails range Slipknot tails range Slipknot loops range N-end length C-end length Type
view details
2.1 25-252 228 1-24 258-258 253-257 24 1 slipknot
view details
3.1 24-257 234 258-258 1-10 11-23 10 0 slipknot
sequence length 258
structure length 258
publication title Rational Engineering of a Mesohalophilic Carbonic Anhydrase to an Extreme Halotolerant Biocatalyst.
pubmed doi rcsb
molecule tags Lyase
molecule keywords CARBONIC ANHYDRASE 2
source organism Bos taurus
total genus Genus: 76
ec nomenclature ec 4.2.1.1: Carbonic anhydrase.
pdb deposition date 2015-05-12
KnotProt deposition date 2018-10-19

pfam database annotations

chain Pfam Accession Code Pfam Family Identifier Pfam Description
A PF00194 Carb_anhydrase Eukaryotic-type carbonic anhydrase
Image from the rcsb pdb (www.rcsb.org)
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similar chains in the KnotProt database (?% sequence similarity)
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similar chains in the pdb database (?% sequence similarity)

 
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