3Q3QA

Crystal structure of spap: an novel alkaline phosphatase from bacterium sphingomonas sp. strain bsar-1
Genus: 191
Slipknot S +31

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Knot core range Knot core length Knot tails range Slipknot tails range Slipknot loops range N-end length C-end length Type
view details
+31 16-316 301 1-15 471-526 317-470 15 56 slipknot
Chain Sequence
AARSIAATPPKLIVAISVDQFSADLFSEYRQYYTGGLKRLTSEGAVFPRGYQSHAATETCPGHSTILTGSRPSRTGIIANNWFDLDAKREDKNLYCAEDESQPGSSSDKYEASPLHLKVPTLGGRMKAANPATRVVSVAGKDRAAIMMGGATADQVWWLGGPQGYVSYKGVAPTPLVTQVNQAFAQRLAQPNPGFELPAQCVSKDFPVQAGNRTVGTGRFARDAGDYKGFRISPEQDAMTLAFAAAAIENMQLGKQAQTDIISIGLSATDYVGHTFGTEGTESCIQVDRLDTELGAFFDKLDKDGIDYVVVLTADHGGHDLPERHRMNAMPMEQRVDMALTPKALNATIAEKAGLPGKKVIWSDGPSGDIYYDKGLTAAQRARVETEALKYLRAHPQVQTVFTKAEIAATPSPSGPPESWSLIQEARASFYPSRSGDLLLLLKPRVMSIPEQAVMGSVATHGSPWDTDRRVPILFWRKGMQHFEQPLGVETVDILPSLAALIKLPVPKDQIDGRCLDLVAGKDDSC
Note, that the numbers in the matrix denote the consecutive residues in the loop, not the index of amino acids in the chain!
Knot K 64x -31

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Knot core range Knot core length Knot tails range Slipknot tails range Slipknot loops range N-end length C-end length Type
- 31 20-119 100 1-19, 120-174 19 55 knot
Fingerprint Knot forming loop Loop type
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> His346 ... Asp300 <->
Bridging ionZn1561
<-> His491 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> His346 ... His304 <->
Bridging ionZn1561
<-> His491 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> Asp345 ... Asp300 <->
Bridging ionZn1561
<-> His491 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> Asp345 ... His304 <->
Bridging ionZn1561
<-> His491 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 <->
Bridging ionCys556
<-> Cys545 ... His491 <->
Bridging ionZn1561
<-> Asp300 ... His346 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31 Ala31 <->
Bridging ionCys556
<-> Cys545 ... His491 <->
Bridging ionZn1561
<-> His304 ... His346 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31 Ala31 <->
Bridging ionCys556
<-> Cys545 ... His491 <->
Bridging ionZn1561
<-> Asp300 ... Asp345 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31 Ala31 <->
Bridging ionCys556
<-> Cys545 ... His491 <->
Bridging ionZn1561
<-> His304 ... Asp345 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> His346 ... Cys314 <-> Cys231 ... Asp300 <->
Bridging ionZn1561
<-> His491 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> His346 ... Asp300 <->
Bridging ionZn1561
<-> His491 ... Val549 <->
Bridging ionCa566
<-> Asp553 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> His346 ... Cys314 <-> Cys231 ... His304 <->
Bridging ionZn1561
<-> His491 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> His346 ... His304 <->
Bridging ionZn1561
<-> His491 ... Val549 <->
Bridging ionCa566
<-> Asp553 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> Asp345 ... Cys314 <-> Cys231 ... Asp300 <->
Bridging ionZn1561
<-> His491 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> Asp345 ... Asp300 <->
Bridging ionZn1561
<-> His491 ... Val549 <->
Bridging ionCa566
<-> Asp553 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> Asp345 ... Cys314 <-> Cys231 ... His304 <->
Bridging ionZn1561
<-> His491 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> Asp345 ... His304 <->
Bridging ionZn1561
<-> His491 ... Val549 <->
Bridging ionCa566
<-> Asp553 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> His346 ... Asp300 <->
Bridging ionZn1561
<-> His491 ... Val549 <->
Bridging ionCa566
<-> Gly551 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> His346 ... His304 <->
Bridging ionZn1561
<-> His491 ... Val549 <->
Bridging ionCa566
<-> Gly551 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> Asp345 ... Asp300 <->
Bridging ionZn1561
<-> His491 ... Val549 <->
Bridging ionCa566
<-> Gly551 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> Asp345 ... His304 <->
Bridging ionZn1561
<-> His491 ... Val549 <->
Bridging ionCa566
<-> Gly551 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Val549 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> Asp300 ... His346 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Val549 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> His304 ... His346 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Val549 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> Asp300 ... Asp345 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Val549 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> His304 ... Asp345 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> His346 ... Asp300 <->
Bridging ionZn1561
<-> His491 ... Gly551 <->
Bridging ionCa566
<-> Asp553 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> His346 ... His304 <->
Bridging ionZn1561
<-> His491 ... Gly551 <->
Bridging ionCa566
<-> Asp553 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> Asp345 ... Asp300 <->
Bridging ionZn1561
<-> His491 ... Gly551 <->
Bridging ionCa566
<-> Asp553 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> Asp345 ... His304 <->
Bridging ionZn1561
<-> His491 ... Gly551 <->
Bridging ionCa566
<-> Asp553 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Asp553 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> Asp300 ... His346 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Asp553 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> His304 ... His346 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Asp553 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> Asp300 ... Asp345 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Asp553 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> His304 ... Asp345 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Gly551 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> Asp300 ... His346 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Gly551 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> His304 ... His346 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Gly551 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> Asp300 ... Asp345 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Gly551 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> His304 ... Asp345 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31 Ala31 <->
Bridging ionCys556
<-> Cys545 ... His491 <->
Bridging ionZn1561
<-> Asp300 ... Cys231 <-> Cys314 ... His346 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31 Ala31 <->
Bridging ionCys556
<-> Cys545 ... His491 <->
Bridging ionZn1561
<-> His304 ... Cys231 <-> Cys314 ... His346 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31 Ala31 <->
Bridging ionCys556
<-> Cys545 ... His491 <->
Bridging ionZn1561
<-> Asp300 ... Cys231 <-> Cys314 ... Asp345 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31 Ala31 <->
Bridging ionCys556
<-> Cys545 ... His491 <->
Bridging ionZn1561
<-> His304 ... Cys231 <-> Cys314 ... Asp345 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> His346 ... Cys314 <-> Cys231 ... Asp300 <->
Bridging ionZn1561
<-> His491 ... Val549 <->
Bridging ionCa566
<-> Asp553 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> His346 ... Cys314 <-> Cys231 ... His304 <->
Bridging ionZn1561
<-> His491 ... Val549 <->
Bridging ionCa566
<-> Asp553 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> Asp345 ... Cys314 <-> Cys231 ... Asp300 <->
Bridging ionZn1561
<-> His491 ... Val549 <->
Bridging ionCa566
<-> Asp553 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> Asp345 ... Cys314 <-> Cys231 ... His304 <->
Bridging ionZn1561
<-> His491 ... Val549 <->
Bridging ionCa566
<-> Asp553 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> His346 ... Cys314 <-> Cys231 ... Asp300 <->
Bridging ionZn1561
<-> His491 ... Val549 <->
Bridging ionCa566
<-> Gly551 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> His346 ... Cys314 <-> Cys231 ... His304 <->
Bridging ionZn1561
<-> His491 ... Val549 <->
Bridging ionCa566
<-> Gly551 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> Asp345 ... Cys314 <-> Cys231 ... Asp300 <->
Bridging ionZn1561
<-> His491 ... Val549 <->
Bridging ionCa566
<-> Gly551 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> Asp345 ... Cys314 <-> Cys231 ... His304 <->
Bridging ionZn1561
<-> His491 ... Val549 <->
Bridging ionCa566
<-> Gly551 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Val549 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> Asp300 ... Cys231 <-> Cys314 ... His346 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Val549 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> His304 ... Cys231 <-> Cys314 ... His346 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Val549 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> Asp300 ... Cys231 <-> Cys314 ... Asp345 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Val549 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> His304 ... Cys231 <-> Cys314 ... Asp345 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> His346 ... Cys314 <-> Cys231 ... Asp300 <->
Bridging ionZn1561
<-> His491 ... Gly551 <->
Bridging ionCa566
<-> Asp553 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> His346 ... Cys314 <-> Cys231 ... His304 <->
Bridging ionZn1561
<-> His491 ... Gly551 <->
Bridging ionCa566
<-> Asp553 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> Asp345 ... Cys314 <-> Cys231 ... Asp300 <->
Bridging ionZn1561
<-> His491 ... Gly551 <->
Bridging ionCa566
<-> Asp553 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31 Ala31 ... Thr89 <->
Bridging ionZn1559
<-> Asp345 ... Cys314 <-> Cys231 ... His304 <->
Bridging ionZn1561
<-> His491 ... Gly551 <->
Bridging ionCa566
<-> Asp553 ...
Chain closureCys556 <-> Ala31
 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Asp553 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> Asp300 ... Cys231 <-> Cys314 ... His346 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Asp553 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> His304 ... Cys231 <-> Cys314 ... His346 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Asp553 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> Asp300 ... Cys231 <-> Cys314 ... Asp345 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Asp553 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> His304 ... Cys231 <-> Cys314 ... Asp345 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Gly551 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> Asp300 ... Cys231 <-> Cys314 ... His346 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Gly551 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> His304 ... Cys231 <-> Cys314 ... His346 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Gly551 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> Asp300 ... Cys231 <-> Cys314 ... Asp345 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
K -31
Chain closureAla31 <-> Cys556
... Gly551 <->
Bridging ionCa566
<-> Asp547 ... His491 <->
Bridging ionZn1561
<-> His304 ... Cys231 <-> Cys314 ... Asp345 <->
Bridging ionZn1559
<-> Thr89 ... Ala31		 probabilistic
Chain Sequence
AARSIAATPPKLIVAISVDQFSADLFSEYRQYYTGGLKRLTSEGAVFPRGYQSHAATETCPGHSTILTGSRPSRTGIIANNWFDLDAKREDKNLYCAEDESQPGSSSDKYEASPLHLKVPTLGGRMKAANPATRVVSVAGKDRAAIMMGGATADQVWWLGGPQGYVSYKGVAPTPLVTQVNQAFAQRLAQPNPGFELPAQCVSKDFPVQAGNRTVGTGRFARDAGDYKGFRISPEQDAMTLAFAAAAIENMQLGKQAQTDIISIGLSATDYVGHTFGTEGTESCIQVDRLDTELGAFFDKLDKDGIDYVVVLTADHGGHDLPERHRMNAMPMEQRVDMALTPKALNATIAEKAGLPGKKVIWSDGPSGDIYYDKGLTAAQRARVETEALKYLRAHPQVQTVFTKAEIAATPSPSGPPESWSLIQEARASFYPSRSGDLLLLLKPRVMSIPEQAVMGSVATHGSPWDTDRRVPILFWRKGMQHFEQPLGVETVDILPSLAALIKLPVPKDQIDGRCLDLVAGKDDSC
Whole chain analysis

  Help Knotoid data interpretation Full screen
Subchain analysis 

Knotoid cutoff: 0.5


Knotoid matrix content: 1


  Help Knotoid subchains data interpretation Full screen
Knot core range Knot core length Knot tails range Slipknot tails range Slipknot loops range N-end length C-end length Type
view details
3.1 17-317 301 1-16 471-526 318-470 16 56 slipknot
view details
2.1 9-470 462 1-8 473-526 471-472 8 54 slipknot
view details
2.1 18-404 387 1-16, 426-526 17-17, 405-425 16 101 slipknot
sequence length 526
structure length 526
publication title X-ray structure reveals a new class and provides insight into evolution of alkaline phosphatases
pubmed doi rcsb
molecule tags Hydrolase
molecule keywords Alkaline phosphatase
source organism Sphingomonas sp.
total genus Genus: 191
ec nomenclature ec 3.1.3.1: Alkaline phosphatase.
pdb deposition date 2010-12-22
KnotProt deposition date 2018-10-19

pfam database annotations

chain Pfam Accession Code Pfam Family Identifier Pfam Description
A PF01663 Phosphodiest Type I phosphodiesterase / nucleotide pyrophosphatase
Image from the rcsb pdb (www.rcsb.org)
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similar chains in the pdb database (?% sequence similarity)

  
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How to cite
KnotProt | Interdisciplinary Laboratory of Biological Systems Modelling