1AJAA

Three-dimensional structure of the d153g mutant of e. coli alkaline phosphatase: a mutant with weaker magnesium binding and increased catalytic activity
Warning
  • Chain breaks within knot 31 (displayed as a gray area on the plot and as '-' on the sequence). The broken part of the chain has been replaced by a straight segment, which may affect what knot types are detected - be careful with interpreting results.
Genus: 158
Slipknot S +31

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Knot core range Knot core length Knot tails range Slipknot tails range Slipknot loops range N-end length C-end length Type
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+31 46-369 324 1-45 419-449 370-418 45 31 slipknot
Chain Sequence
TPEMPVLENRAAQGDITAPGGARRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKAAGLATGNVSTAELQGATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAETATAGEWQGKTLREQAEARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGAS------AANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADHAHASQIVAPDTKAPGLTQALNTKDGAVMVMSYEHTGSQ--------LRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK
Whole chain analysis

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Subchain analysis 

Knotoid cutoff: 0.5


Knotoid matrix content: 1


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Knot core range Knot core length Knot tails range Slipknot tails range Slipknot loops range N-end length C-end length Type
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2.1 47-365 319 1-46 402-435 366-401 46 34 slipknot
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2.1 44-401 358 1-43 408-435 402-407 43 28 slipknot
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2.1 16-360 345 1-7, 366-435 8-15, 361-365 7 70 slipknot
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3.1 28-360 333 1-15, 366-435 16-27, 361-365 15 70 slipknot
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2.1 29-360 332 1-27, 366-435 28-28, 361-365 27 70 slipknot
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2.1 30-360 331 1-28, 366-435 29-29, 361-365 28 70 slipknot
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2.1 36-361 326 1-29, 366-435 30-35, 362-365 29 70 slipknot
sequence length 449
structure length 435
publication title Crystallographic analysis of reversible metal binding observed in a mutant (Asp153-->Gly) of Escherichia coli alkaline phosphatase.
pubmed doi rcsb
molecule tags Non specific mono-esterase
molecule keywords ALKALINE PHOSPHATASE
source organism Escherichia coli
missing residues 326-331, 403-410
total genus Genus: 158
ec nomenclature ec 3.1.3.1: Alkaline phosphatase.
pdb deposition date 1995-08-19
KnotProt deposition date 2014-07-31

pfam database annotations

chain Pfam Accession Code Pfam Family Identifier Pfam Description
A PF00245 Alk_phosphatase Alkaline phosphatase
Image from the rcsb pdb (www.rcsb.org)
cath code
ClassArchitectureTopologyHomologyDomain
3.40.720.10 Alpha Beta 3-Layer(aba) Sandwich Alkaline Phosphatase, subunit A Alkaline Phosphatase, subunit A 1ajaA00
1ZEBA 1AJAA 1ALJA 2GLQA 3TWZA 1KH4A 1KH7A 1ANJA 2GA3A 1KHKA 3NVLA 3UN5A 3M7VA 1N2KA 1ELYA 1AUKA 1E1ZP 1O99A 3OT9A 1KH5A 3M8ZA 1HDHA 1URBA 3SZZA 1K7HA 1ELZA 2QZUA 1EI6A 1HJKA 1KHLA 1FSUA 3T01A 1E2SP 1ZEDA 1AJCA 1SHQA 3CMRA 1EQJA 1EW2A 3UN3A 3IGZB 1B8JA 2IFYA 3SZYA 1ED8A 1ANIA 2ANHA 1ZEFA 3UO0A 1KH9A 3TX0A 3MK2A 4FDIA 3M8YA 1ALIA 4FDJA 1E3CP 1EW9A 3UNYA 2G9YA 1ALKA 3ED4A 3DPCA 1ELXA 1EW8A 1O98A 1AJDA 1AJBA 3T02A 3T00A 1N2LA 3MK0A 1EJJA 1Y6VA 3BDFA 1URAA 1KHNA 1Y7AA 3M8WA 3MK1A 3UN2A 1E33P 1P49A 3DYCA 1HQAA 1ED9A 1SHNA 3BDGA 3BDHA 3IGYB 1KHJA 3BDGB
chains in the KnotProt database with same CATH superfamily
1ZEBA 1AJAA 1ALJA 2GLQA 3TWZA 1KH4A 1KH7A 1ANJA 2GA3A 1KHKA 3NVLA 3UN5A 3M7VA 1N2KA 1ELYA 1AUKA 1E1ZP 1O99A 3OT9A 1KH5A 3M8ZA 1HDHA 1URBA 3SZZA 1K7HA 1ELZA 2QZUA 1EI6A 1HJKA 1KHLA 1FSUA 3T01A 1E2SP 1ZEDA 1AJCA 1SHQA 3CMRA 1EQJA 1EW2A 3UN3A 3IGZB 1B8JA 2IFYA 3SZYA 1ED8A 1ANIA 2ANHA 1ZEFA 3UO0A 1KH9A 3TX0A 3MK2A 4FDIA 3M8YA 1ALIA 4FDJA 1E3CP 1EW9A 3UNYA 2G9YA 1ALKA 3ED4A 3DPCA 1ELXA 1EW8A 1O98A 1AJDA 1AJBA 3T02A 3T00A 1N2LA 3MK0A 1EJJA 1Y6VA 3BDFA 1URAA 1KHNA 1Y7AA 3M8WA 3MK1A 3UN2A 1E33P 1P49A 3DYCA 1HQAA 1ED9A 1SHNA 3BDGA 3BDHA 3IGYB 1KHJA 3BDGB
chains in the KnotProt database with same CATH topology
1ZEBA 1AJAA 1ALJA 2GLQA 3TWZA 1KH4A 1KH7A 1ANJA 2GA3A 1KHKA 3NVLA 3UN5A 3M7VA 1N2KA 1ELYA 1AUKA 1E1ZP 1O99A 3OT9A 1KH5A 3M8ZA 1HDHA 1URBA 3SZZA 1K7HA 1ELZA 2QZUA 1EI6A 1HJKA 1KHLA 1FSUA 3T01A 1E2SP 1ZEDA 1AJCA 1SHQA 3CMRA 1EQJA 1EW2A 3UN3A 3IGZB 1B8JA 2IFYA 3SZYA 1ED8A 1ANIA 2ANHA 1ZEFA 3UO0A 1KH9A 3TX0A 3MK2A 4FDIA 3M8YA 1ALIA 4FDJA 1E3CP 1EW9A 3UNYA 2G9YA 1ALKA 3ED4A 3DPCA 1ELXA 1EW8A 1O98A 1AJDA 1AJBA 3T02A 3T00A 1N2LA 3MK0A 1EJJA 1Y6VA 3BDFA 1URAA 1KHNA 1Y7AA 3M8WA 3MK1A 3UN2A 1E33P 1P49A 3DYCA 1HQAA 1ED9A 1SHNA 3BDGA 3BDHA 3IGYB 1KHJA 3BDGB
chains in the KnotProt database with same CATH homology

  
#chains in the KnotProt database with same CATH superfamily
 1ZEB A;  1AJA A;  1ALJ A;  2GLQ A;  3TWZ A;  1KH4 A;  1KH7 A;  1ANJ A;  2GA3 A;  1KHK A;  3NVL A;  3UN5 A;  3M7V A;  1N2K A;  1ELY A;  1AUK A;  1E1Z P;  1O99 A;  3OT9 A;  1KH5 A;  3M8Z A;  1HDH A;  1URB A;  3SZZ A;  1K7H A;  1ELZ A;  2QZU A;  1EI6 A;  1HJK A;  1KHL A;  1FSU A;  3T01 A;  1E2S P;  1ZED A;  1AJC A;  1SHQ A;  3CMR A;  1EQJ A;  1EW2 A;  3UN3 A;  3IGZ B;  1B8J A;  2IFY A;  3SZY A;  1ED8 A;  1ANI A;  2ANH A;  1ZEF A;  3UO0 A;  1KH9 A;  3TX0 A;  3MK2 A;  4FDI A;  3M8Y A;  1ALI A;  4FDJ A;  1E3C P;  1EW9 A;  3UNY A;  2G9Y A;  1ALK A;  3ED4 A;  3DPC A;  1ELX A;  1EW8 A;  1O98 A;  1AJD A;  1AJB A;  3T02 A;  3T00 A;  1N2L A;  3MK0 A;  1EJJ A;  1Y6V A;  3BDF A;  1URA A;  1KHN A;  1Y7A A;  3M8W A;  3MK1 A;  3UN2 A;  1E33 P;  1P49 A;  3DYC A;  1HQA A;  1ED9 A;  1SHN A;  3BDG A;  3BDH A;  3IGY B;  1KHJ A;  3BDG B; 
#chains in the KnotProt database with same CATH topology
 1ZEB A;  1AJA A;  1ALJ A;  2GLQ A;  3TWZ A;  1KH4 A;  1KH7 A;  1ANJ A;  2GA3 A;  1KHK A;  3NVL A;  3UN5 A;  3M7V A;  1N2K A;  1ELY A;  1AUK A;  1E1Z P;  1O99 A;  3OT9 A;  1KH5 A;  3M8Z A;  1HDH A;  1URB A;  3SZZ A;  1K7H A;  1ELZ A;  2QZU A;  1EI6 A;  1HJK A;  1KHL A;  1FSU A;  3T01 A;  1E2S P;  1ZED A;  1AJC A;  1SHQ A;  3CMR A;  1EQJ A;  1EW2 A;  3UN3 A;  3IGZ B;  1B8J A;  2IFY A;  3SZY A;  1ED8 A;  1ANI A;  2ANH A;  1ZEF A;  3UO0 A;  1KH9 A;  3TX0 A;  3MK2 A;  4FDI A;  3M8Y A;  1ALI A;  4FDJ A;  1E3C P;  1EW9 A;  3UNY A;  2G9Y A;  1ALK A;  3ED4 A;  3DPC A;  1ELX A;  1EW8 A;  1O98 A;  1AJD A;  1AJB A;  3T02 A;  3T00 A;  1N2L A;  3MK0 A;  1EJJ A;  1Y6V A;  3BDF A;  1URA A;  1KHN A;  1Y7A A;  3M8W A;  3MK1 A;  3UN2 A;  1E33 P;  1P49 A;  3DYC A;  1HQA A;  1ED9 A;  1SHN A;  3BDG A;  3BDH A;  3IGY B;  1KHJ A;  3BDG B; 
#chains in the KnotProt database with same CATH homology
 1ZEB A;  1AJA A;  1ALJ A;  2GLQ A;  3TWZ A;  1KH4 A;  1KH7 A;  1ANJ A;  2GA3 A;  1KHK A;  3NVL A;  3UN5 A;  3M7V A;  1N2K A;  1ELY A;  1AUK A;  1E1Z P;  1O99 A;  3OT9 A;  1KH5 A;  3M8Z A;  1HDH A;  1URB A;  3SZZ A;  1K7H A;  1ELZ A;  2QZU A;  1EI6 A;  1HJK A;  1KHL A;  1FSU A;  3T01 A;  1E2S P;  1ZED A;  1AJC A;  1SHQ A;  3CMR A;  1EQJ A;  1EW2 A;  3UN3 A;  3IGZ B;  1B8J A;  2IFY A;  3SZY A;  1ED8 A;  1ANI A;  2ANH A;  1ZEF A;  3UO0 A;  1KH9 A;  3TX0 A;  3MK2 A;  4FDI A;  3M8Y A;  1ALI A;  4FDJ A;  1E3C P;  1EW9 A;  3UNY A;  2G9Y A;  1ALK A;  3ED4 A;  3DPC A;  1ELX A;  1EW8 A;  1O98 A;  1AJD A;  1AJB A;  3T02 A;  3T00 A;  1N2L A;  3MK0 A;  1EJJ A;  1Y6V A;  3BDF A;  1URA A;  1KHN A;  1Y7A A;  3M8W A;  3MK1 A;  3UN2 A;  1E33 P;  1P49 A;  3DYC A;  1HQA A;  1ED9 A;  1SHN A;  3BDG A;  3BDH A;  3IGY B;  1KHJ A;  3BDG B;
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similar chains in the KnotProt database (?% sequence similarity)
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similar chains in the pdb database (?% sequence similarity)

  
#similar chains in the KnotProt database (?% sequence similarity)
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#similar chains, but unknotted
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#similar chains in the pdb database (?% sequence similarity)
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