1KHKA

E. coli alkaline phosphatase mutant (d153hd330n)
Warning
  • Chain breaks within knotoid 31 (displayed as a gray area on the plot and as '-' on the sequence). The broken part of the chain has been replaced by a straight segment, which may affect what knot types are detected - be careful with interpreting results.
Genus: 149
Slipknot S +31

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Knot core range Knot core length Knot tails range Slipknot tails range Slipknot loops range N-end length C-end length Type
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+31 42-363 322 1-41 414-444 364-413 41 31 slipknot
Chain Sequence
VLENRAAQGNITAPGGARRLTGDQTAALRNSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKAAGLATGNVSTAELQHATPAALVAHVTSRKCYGPSATSQKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAETATAGEWQGKTLREEAEARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQNHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADHAHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK
Whole chain analysis

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Subchain analysis 

Knotoid cutoff: 0.5


Knotoid matrix content: 1


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Knot core range Knot core length Knot tails range Slipknot tails range Slipknot loops range N-end length C-end length Type
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2.1 42-366 325 1-41 398-444 367-397 41 47 slipknot
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2.1 39-408 370 1-7, 416-444 8-38, 409-415 7 29 slipknot
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2.1 27-362 336 1-19, 367-444 20-26, 363-366 19 78 slipknot
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2.1 31-362 332 1-26, 367-444 27-30, 363-366 26 78 slipknot
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2.1 36-362 327 1-30, 367-444 31-35, 363-366 30 78 slipknot
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3.1 40-364 325 1-35, 367-444 36-39, 365-366 35 78 slipknot
sequence length 444
structure length 444
publication title Artificial evolution of an enzyme active site: structural studies of three highly active mutants of Escherichia coli alkaline phosphatase.
pubmed doi rcsb
molecule tags Hydrolase
molecule keywords Alkaline Phosphatase
source organism Escherichia coli
total genus Genus: 149
ec nomenclature ec 3.1.3.1: Alkaline phosphatase.
pdb deposition date 2001-11-30
KnotProt deposition date 2014-07-31

pfam database annotations

chain Pfam Accession Code Pfam Family Identifier Pfam Description
A PF00245 Alk_phosphatase Alkaline phosphatase
Image from the rcsb pdb (www.rcsb.org)
cath code
ClassArchitectureTopologyHomologyDomain
3.40.720.10 Alpha Beta 3-Layer(aba) Sandwich Alkaline Phosphatase, subunit A Alkaline Phosphatase, subunit A 1khkA00
3UNYA 1ZEBA 3UN2A 1EJJA 3OT9A 1KH4A 3BDHA 3SZYA 1B8JA 1ELXA 3MK0A 3BDGA 1E3CP 4FDJA 1EW8A 1ZEFA 1N2KA 1KHNA 3UO0A 2G9YA 3TX0A 1ANJA 1E33P 1URBA 3IGYB 1AJDA 1KH5A 1EW2A 2GA3A 1K7HA 1EW9A 1E2SP 3UN3A 1E1ZP 1ED9A 1FSUA 1AJCA 1O99A 1SHNA 2IFYA 1ALKA 1ELYA 3T02A 1ED8A 1ANIA 1AJBA 3M8WA 3IGZB 1ALIA 1EQJA 1EI6A 3DYCA 1ALJA 3MK1A 1SHQA 3M8ZA 3SZZA 1URAA 1ELZA 3M8YA 3T00A 3BDGB 1HQAA 3ED4A 1P49A 3NVLA 1KHLA 1KHKA 4FDIA 1N2LA 1HJKA 2GLQA 3T01A 1Y6VA 3BDFA 3DPCA 1O98A 3MK2A 1KHJA 3M7VA 3UN5A 3TWZA 3CMRA 1HDHA 1Y7AA 1ZEDA 1KH7A 1AJAA 1AUKA 1KH9A 2QZUA 2ANHA
chains in the KnotProt database with same CATH superfamily
3UNYA 1ZEBA 3UN2A 1EJJA 3OT9A 1KH4A 3BDHA 3SZYA 1B8JA 1ELXA 3MK0A 3BDGA 1E3CP 4FDJA 1EW8A 1ZEFA 1N2KA 1KHNA 3UO0A 2G9YA 3TX0A 1ANJA 1E33P 1URBA 3IGYB 1AJDA 1KH5A 1EW2A 2GA3A 1K7HA 1EW9A 1E2SP 3UN3A 1E1ZP 1ED9A 1FSUA 1AJCA 1O99A 1SHNA 2IFYA 1ALKA 1ELYA 3T02A 1ED8A 1ANIA 1AJBA 3M8WA 3IGZB 1ALIA 1EQJA 1EI6A 3DYCA 1ALJA 3MK1A 1SHQA 3M8ZA 3SZZA 1URAA 1ELZA 3M8YA 3T00A 3BDGB 1HQAA 3ED4A 1P49A 3NVLA 1KHLA 1KHKA 4FDIA 1N2LA 1HJKA 2GLQA 3T01A 1Y6VA 3BDFA 3DPCA 1O98A 3MK2A 1KHJA 3M7VA 3UN5A 3TWZA 3CMRA 1HDHA 1Y7AA 1ZEDA 1KH7A 1AJAA 1AUKA 1KH9A 2QZUA 2ANHA
chains in the KnotProt database with same CATH topology
3UNYA 1ZEBA 3UN2A 1EJJA 3OT9A 1KH4A 3BDHA 3SZYA 1B8JA 1ELXA 3MK0A 3BDGA 1E3CP 4FDJA 1EW8A 1ZEFA 1N2KA 1KHNA 3UO0A 2G9YA 3TX0A 1ANJA 1E33P 1URBA 3IGYB 1AJDA 1KH5A 1EW2A 2GA3A 1K7HA 1EW9A 1E2SP 3UN3A 1E1ZP 1ED9A 1FSUA 1AJCA 1O99A 1SHNA 2IFYA 1ALKA 1ELYA 3T02A 1ED8A 1ANIA 1AJBA 3M8WA 3IGZB 1ALIA 1EQJA 1EI6A 3DYCA 1ALJA 3MK1A 1SHQA 3M8ZA 3SZZA 1URAA 1ELZA 3M8YA 3T00A 3BDGB 1HQAA 3ED4A 1P49A 3NVLA 1KHLA 1KHKA 4FDIA 1N2LA 1HJKA 2GLQA 3T01A 1Y6VA 3BDFA 3DPCA 1O98A 3MK2A 1KHJA 3M7VA 3UN5A 3TWZA 3CMRA 1HDHA 1Y7AA 1ZEDA 1KH7A 1AJAA 1AUKA 1KH9A 2QZUA 2ANHA
chains in the KnotProt database with same CATH homology

  
#chains in the KnotProt database with same CATH superfamily
 3UNY A;  1ZEB A;  3UN2 A;  1EJJ A;  3OT9 A;  1KH4 A;  3BDH A;  3SZY A;  1B8J A;  1ELX A;  3MK0 A;  3BDG A;  1E3C P;  4FDJ A;  1EW8 A;  1ZEF A;  1N2K A;  1KHN A;  3UO0 A;  2G9Y A;  3TX0 A;  1ANJ A;  1E33 P;  1URB A;  3IGY B;  1AJD A;  1KH5 A;  1EW2 A;  2GA3 A;  1K7H A;  1EW9 A;  1E2S P;  3UN3 A;  1E1Z P;  1ED9 A;  1FSU A;  1AJC A;  1O99 A;  1SHN A;  2IFY A;  1ALK A;  1ELY A;  3T02 A;  1ED8 A;  1ANI A;  1AJB A;  3M8W A;  3IGZ B;  1ALI A;  1EQJ A;  1EI6 A;  3DYC A;  1ALJ A;  3MK1 A;  1SHQ A;  3M8Z A;  3SZZ A;  1URA A;  1ELZ A;  3M8Y A;  3T00 A;  3BDG B;  1HQA A;  3ED4 A;  1P49 A;  3NVL A;  1KHL A;  1KHK A;  4FDI A;  1N2L A;  1HJK A;  2GLQ A;  3T01 A;  1Y6V A;  3BDF A;  3DPC A;  1O98 A;  3MK2 A;  1KHJ A;  3M7V A;  3UN5 A;  3TWZ A;  3CMR A;  1HDH A;  1Y7A A;  1ZED A;  1KH7 A;  1AJA A;  1AUK A;  1KH9 A;  2QZU A;  2ANH A; 
#chains in the KnotProt database with same CATH topology
 3UNY A;  1ZEB A;  3UN2 A;  1EJJ A;  3OT9 A;  1KH4 A;  3BDH A;  3SZY A;  1B8J A;  1ELX A;  3MK0 A;  3BDG A;  1E3C P;  4FDJ A;  1EW8 A;  1ZEF A;  1N2K A;  1KHN A;  3UO0 A;  2G9Y A;  3TX0 A;  1ANJ A;  1E33 P;  1URB A;  3IGY B;  1AJD A;  1KH5 A;  1EW2 A;  2GA3 A;  1K7H A;  1EW9 A;  1E2S P;  3UN3 A;  1E1Z P;  1ED9 A;  1FSU A;  1AJC A;  1O99 A;  1SHN A;  2IFY A;  1ALK A;  1ELY A;  3T02 A;  1ED8 A;  1ANI A;  1AJB A;  3M8W A;  3IGZ B;  1ALI A;  1EQJ A;  1EI6 A;  3DYC A;  1ALJ A;  3MK1 A;  1SHQ A;  3M8Z A;  3SZZ A;  1URA A;  1ELZ A;  3M8Y A;  3T00 A;  3BDG B;  1HQA A;  3ED4 A;  1P49 A;  3NVL A;  1KHL A;  1KHK A;  4FDI A;  1N2L A;  1HJK A;  2GLQ A;  3T01 A;  1Y6V A;  3BDF A;  3DPC A;  1O98 A;  3MK2 A;  1KHJ A;  3M7V A;  3UN5 A;  3TWZ A;  3CMR A;  1HDH A;  1Y7A A;  1ZED A;  1KH7 A;  1AJA A;  1AUK A;  1KH9 A;  2QZU A;  2ANH A; 
#chains in the KnotProt database with same CATH homology
 3UNY A;  1ZEB A;  3UN2 A;  1EJJ A;  3OT9 A;  1KH4 A;  3BDH A;  3SZY A;  1B8J A;  1ELX A;  3MK0 A;  3BDG A;  1E3C P;  4FDJ A;  1EW8 A;  1ZEF A;  1N2K A;  1KHN A;  3UO0 A;  2G9Y A;  3TX0 A;  1ANJ A;  1E33 P;  1URB A;  3IGY B;  1AJD A;  1KH5 A;  1EW2 A;  2GA3 A;  1K7H A;  1EW9 A;  1E2S P;  3UN3 A;  1E1Z P;  1ED9 A;  1FSU A;  1AJC A;  1O99 A;  1SHN A;  2IFY A;  1ALK A;  1ELY A;  3T02 A;  1ED8 A;  1ANI A;  1AJB A;  3M8W A;  3IGZ B;  1ALI A;  1EQJ A;  1EI6 A;  3DYC A;  1ALJ A;  3MK1 A;  1SHQ A;  3M8Z A;  3SZZ A;  1URA A;  1ELZ A;  3M8Y A;  3T00 A;  3BDG B;  1HQA A;  3ED4 A;  1P49 A;  3NVL A;  1KHL A;  1KHK A;  4FDI A;  1N2L A;  1HJK A;  2GLQ A;  3T01 A;  1Y6V A;  3BDF A;  3DPC A;  1O98 A;  3MK2 A;  1KHJ A;  3M7V A;  3UN5 A;  3TWZ A;  3CMR A;  1HDH A;  1Y7A A;  1ZED A;  1KH7 A;  1AJA A;  1AUK A;  1KH9 A;  2QZU A;  2ANH A;
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similar chains in the KnotProt database (?% sequence similarity)
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similar chains in the pdb database (?% sequence similarity)

  
#similar chains in the KnotProt database (?% sequence similarity)
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#similar chains, but unknotted
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#similar chains in the pdb database (?% sequence similarity)
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KnotProt | Interdisciplinary Laboratory of Biological Systems Modelling