1KHKA

E. coli alkaline phosphatase mutant (d153hd330n)
Warning
  • Chain breaks within knotoid 31 (displayed as a gray area on the plot and as '-' on the sequence). The broken part of the chain has been replaced by a straight segment, which may affect what knot types are detected - be careful with interpreting results.
Genus: 149
Slipknot S +31

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Knot core range Knot core length Knot tails range Slipknot tails range Slipknot loops range N-end length C-end length Type
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+31 42-363 322 1-41 414-444 364-413 41 31 slipknot
Chain Sequence
VLENRAAQGNITAPGGARRLTGDQTAALRNSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKAAGLATGNVSTAELQHATPAALVAHVTSRKCYGPSATSQKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAETATAGEWQGKTLREEAEARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQNHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADHAHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK
Whole chain analysis

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Subchain analysis 

Knotoid cutoff: 0.5


Knotoid matrix content: 1


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Knot core range Knot core length Knot tails range Slipknot tails range Slipknot loops range N-end length C-end length Type
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2.1 42-366 325 1-41 398-444 367-397 41 47 slipknot
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2.1 39-408 370 1-7, 416-444 8-38, 409-415 7 29 slipknot
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2.1 27-362 336 1-19, 367-444 20-26, 363-366 19 78 slipknot
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2.1 31-362 332 1-26, 367-444 27-30, 363-366 26 78 slipknot
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2.1 36-362 327 1-30, 367-444 31-35, 363-366 30 78 slipknot
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3.1 40-364 325 1-35, 367-444 36-39, 365-366 35 78 slipknot
sequence length 444
structure length 444
publication title Artificial evolution of an enzyme active site: structural studies of three highly active mutants of Escherichia coli alkaline phosphatase.
pubmed doi rcsb
molecule tags Hydrolase
molecule keywords Alkaline Phosphatase
source organism Escherichia coli
total genus Genus: 149
ec nomenclature ec 3.1.3.1: Alkaline phosphatase.
pdb deposition date 2001-11-30
KnotProt deposition date 2014-07-31

pfam database annotations

chain Pfam Accession Code Pfam Family Identifier Pfam Description
A PF00245 Alk_phosphatase Alkaline phosphatase
Image from the rcsb pdb (www.rcsb.org)
cath code
ClassArchitectureTopologyHomologyDomain
3.40.720.10 Alpha Beta 3-Layer(aba) Sandwich Alkaline Phosphatase, subunit A Alkaline Phosphatase, subunit A 1khkA00
3BDHA 3MK2A 1N2KA 3DYCA 1ED8A 1ALKA 1KHNA 3TWZA 1AJAA 1B8JA 1Y6VA 1N2LA 3T00A 3DPCA 3T02A 1EW8A 4FDIA 3OT9A 1EW9A 3ED4A 3T01A 1O98A 3CMRA 1EQJA 1EI6A 1EJJA 1ZEDA 1ELXA 1P49A 1KH9A 3M7VA 1ZEFA 1E2SP 1KHJA 1E33P 1KH7A 1KH4A 1HDHA 1HJKA 1E1ZP 3UN2A 1KHKA 2G9YA 3M8ZA 3TX0A 3UN5A 1ZEBA 2QZUA 3SZYA 3BDGA 1HQAA 3SZZA 1ALJA 3UO0A 3M8YA 3MK1A 1AUKA 1K7HA 1ED9A 1EW2A 1SHNA 1ANIA 1ANJA 4FDJA 1URAA 1FSUA 1Y7AA 3MK0A 1SHQA 3IGYB 3BDGB 1ELYA 2GA3A 3M8WA 1KHLA 1AJDA 3IGZB 2GLQA 3BDFA 1AJBA 3NVLA 1E3CP 1ALIA 3UNYA 1O99A 3UN3A 2IFYA 1URBA 2ANHA 1KH5A 1AJCA 1ELZA
chains in the KnotProt database with same CATH superfamily
3BDHA 3MK2A 1N2KA 3DYCA 1ED8A 1ALKA 1KHNA 3TWZA 1AJAA 1B8JA 1Y6VA 1N2LA 3T00A 3DPCA 3T02A 1EW8A 4FDIA 3OT9A 1EW9A 3ED4A 3T01A 1O98A 3CMRA 1EQJA 1EI6A 1EJJA 1ZEDA 1ELXA 1P49A 1KH9A 3M7VA 1ZEFA 1E2SP 1KHJA 1E33P 1KH7A 1KH4A 1HDHA 1HJKA 1E1ZP 3UN2A 1KHKA 2G9YA 3M8ZA 3TX0A 3UN5A 1ZEBA 2QZUA 3SZYA 3BDGA 1HQAA 3SZZA 1ALJA 3UO0A 3M8YA 3MK1A 1AUKA 1K7HA 1ED9A 1EW2A 1SHNA 1ANIA 1ANJA 4FDJA 1URAA 1FSUA 1Y7AA 3MK0A 1SHQA 3IGYB 3BDGB 1ELYA 2GA3A 3M8WA 1KHLA 1AJDA 3IGZB 2GLQA 3BDFA 1AJBA 3NVLA 1E3CP 1ALIA 3UNYA 1O99A 3UN3A 2IFYA 1URBA 2ANHA 1KH5A 1AJCA 1ELZA
chains in the KnotProt database with same CATH topology
3BDHA 3MK2A 1N2KA 3DYCA 1ED8A 1ALKA 1KHNA 3TWZA 1AJAA 1B8JA 1Y6VA 1N2LA 3T00A 3DPCA 3T02A 1EW8A 4FDIA 3OT9A 1EW9A 3ED4A 3T01A 1O98A 3CMRA 1EQJA 1EI6A 1EJJA 1ZEDA 1ELXA 1P49A 1KH9A 3M7VA 1ZEFA 1E2SP 1KHJA 1E33P 1KH7A 1KH4A 1HDHA 1HJKA 1E1ZP 3UN2A 1KHKA 2G9YA 3M8ZA 3TX0A 3UN5A 1ZEBA 2QZUA 3SZYA 3BDGA 1HQAA 3SZZA 1ALJA 3UO0A 3M8YA 3MK1A 1AUKA 1K7HA 1ED9A 1EW2A 1SHNA 1ANIA 1ANJA 4FDJA 1URAA 1FSUA 1Y7AA 3MK0A 1SHQA 3IGYB 3BDGB 1ELYA 2GA3A 3M8WA 1KHLA 1AJDA 3IGZB 2GLQA 3BDFA 1AJBA 3NVLA 1E3CP 1ALIA 3UNYA 1O99A 3UN3A 2IFYA 1URBA 2ANHA 1KH5A 1AJCA 1ELZA
chains in the KnotProt database with same CATH homology

  
#chains in the KnotProt database with same CATH superfamily
 3BDH A;  3MK2 A;  1N2K A;  3DYC A;  1ED8 A;  1ALK A;  1KHN A;  3TWZ A;  1AJA A;  1B8J A;  1Y6V A;  1N2L A;  3T00 A;  3DPC A;  3T02 A;  1EW8 A;  4FDI A;  3OT9 A;  1EW9 A;  3ED4 A;  3T01 A;  1O98 A;  3CMR A;  1EQJ A;  1EI6 A;  1EJJ A;  1ZED A;  1ELX A;  1P49 A;  1KH9 A;  3M7V A;  1ZEF A;  1E2S P;  1KHJ A;  1E33 P;  1KH7 A;  1KH4 A;  1HDH A;  1HJK A;  1E1Z P;  3UN2 A;  1KHK A;  2G9Y A;  3M8Z A;  3TX0 A;  3UN5 A;  1ZEB A;  2QZU A;  3SZY A;  3BDG A;  1HQA A;  3SZZ A;  1ALJ A;  3UO0 A;  3M8Y A;  3MK1 A;  1AUK A;  1K7H A;  1ED9 A;  1EW2 A;  1SHN A;  1ANI A;  1ANJ A;  4FDJ A;  1URA A;  1FSU A;  1Y7A A;  3MK0 A;  1SHQ A;  3IGY B;  3BDG B;  1ELY A;  2GA3 A;  3M8W A;  1KHL A;  1AJD A;  3IGZ B;  2GLQ A;  3BDF A;  1AJB A;  3NVL A;  1E3C P;  1ALI A;  3UNY A;  1O99 A;  3UN3 A;  2IFY A;  1URB A;  2ANH A;  1KH5 A;  1AJC A;  1ELZ A; 
#chains in the KnotProt database with same CATH topology
 3BDH A;  3MK2 A;  1N2K A;  3DYC A;  1ED8 A;  1ALK A;  1KHN A;  3TWZ A;  1AJA A;  1B8J A;  1Y6V A;  1N2L A;  3T00 A;  3DPC A;  3T02 A;  1EW8 A;  4FDI A;  3OT9 A;  1EW9 A;  3ED4 A;  3T01 A;  1O98 A;  3CMR A;  1EQJ A;  1EI6 A;  1EJJ A;  1ZED A;  1ELX A;  1P49 A;  1KH9 A;  3M7V A;  1ZEF A;  1E2S P;  1KHJ A;  1E33 P;  1KH7 A;  1KH4 A;  1HDH A;  1HJK A;  1E1Z P;  3UN2 A;  1KHK A;  2G9Y A;  3M8Z A;  3TX0 A;  3UN5 A;  1ZEB A;  2QZU A;  3SZY A;  3BDG A;  1HQA A;  3SZZ A;  1ALJ A;  3UO0 A;  3M8Y A;  3MK1 A;  1AUK A;  1K7H A;  1ED9 A;  1EW2 A;  1SHN A;  1ANI A;  1ANJ A;  4FDJ A;  1URA A;  1FSU A;  1Y7A A;  3MK0 A;  1SHQ A;  3IGY B;  3BDG B;  1ELY A;  2GA3 A;  3M8W A;  1KHL A;  1AJD A;  3IGZ B;  2GLQ A;  3BDF A;  1AJB A;  3NVL A;  1E3C P;  1ALI A;  3UNY A;  1O99 A;  3UN3 A;  2IFY A;  1URB A;  2ANH A;  1KH5 A;  1AJC A;  1ELZ A; 
#chains in the KnotProt database with same CATH homology
 3BDH A;  3MK2 A;  1N2K A;  3DYC A;  1ED8 A;  1ALK A;  1KHN A;  3TWZ A;  1AJA A;  1B8J A;  1Y6V A;  1N2L A;  3T00 A;  3DPC A;  3T02 A;  1EW8 A;  4FDI A;  3OT9 A;  1EW9 A;  3ED4 A;  3T01 A;  1O98 A;  3CMR A;  1EQJ A;  1EI6 A;  1EJJ A;  1ZED A;  1ELX A;  1P49 A;  1KH9 A;  3M7V A;  1ZEF A;  1E2S P;  1KHJ A;  1E33 P;  1KH7 A;  1KH4 A;  1HDH A;  1HJK A;  1E1Z P;  3UN2 A;  1KHK A;  2G9Y A;  3M8Z A;  3TX0 A;  3UN5 A;  1ZEB A;  2QZU A;  3SZY A;  3BDG A;  1HQA A;  3SZZ A;  1ALJ A;  3UO0 A;  3M8Y A;  3MK1 A;  1AUK A;  1K7H A;  1ED9 A;  1EW2 A;  1SHN A;  1ANI A;  1ANJ A;  4FDJ A;  1URA A;  1FSU A;  1Y7A A;  3MK0 A;  1SHQ A;  3IGY B;  3BDG B;  1ELY A;  2GA3 A;  3M8W A;  1KHL A;  1AJD A;  3IGZ B;  2GLQ A;  3BDF A;  1AJB A;  3NVL A;  1E3C P;  1ALI A;  3UNY A;  1O99 A;  3UN3 A;  2IFY A;  1URB A;  2ANH A;  1KH5 A;  1AJC A;  1ELZ A;
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similar chains in the KnotProt database (?% sequence similarity)
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similar chains in the pdb database (?% sequence similarity)

  
#similar chains in the KnotProt database (?% sequence similarity)
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#similar chains, but unknotted
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#similar chains in the pdb database (?% sequence similarity)
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