1AJAA

Three-dimensional structure of the d153g mutant of e. coli alkaline phosphatase: a mutant with weaker magnesium binding and increased catalytic activity
Warning
  • Chain breaks within knot 31 (displayed as a gray area on the plot and as '-' on the sequence). The broken part of the chain has been replaced by a straight segment, which may affect what knot types are detected - be careful with interpreting results.
Genus: 158
Slipknot S +31

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Knot core range Knot core length Knot tails range Slipknot tails range Slipknot loops range N-end length C-end length Type
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+31 46-369 324 1-45 419-449 370-418 45 31 slipknot
Chain Sequence
TPEMPVLENRAAQGDITAPGGARRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKAAGLATGNVSTAELQGATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAETATAGEWQGKTLREQAEARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGAS------AANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADHAHASQIVAPDTKAPGLTQALNTKDGAVMVMSYEHTGSQ--------LRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK
Whole chain analysis

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Subchain analysis 

Knotoid cutoff: 0.5


Knotoid matrix content: 1


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Knot core range Knot core length Knot tails range Slipknot tails range Slipknot loops range N-end length C-end length Type
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2.1 47-365 319 1-46 402-435 366-401 46 34 slipknot
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2.1 44-401 358 1-43 408-435 402-407 43 28 slipknot
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2.1 16-360 345 1-7, 366-435 8-15, 361-365 7 70 slipknot
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3.1 28-360 333 1-15, 366-435 16-27, 361-365 15 70 slipknot
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2.1 29-360 332 1-27, 366-435 28-28, 361-365 27 70 slipknot
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2.1 30-360 331 1-28, 366-435 29-29, 361-365 28 70 slipknot
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2.1 36-361 326 1-29, 366-435 30-35, 362-365 29 70 slipknot
sequence length 449
structure length 435
publication title Crystallographic analysis of reversible metal binding observed in a mutant (Asp153-->Gly) of Escherichia coli alkaline phosphatase.
pubmed doi rcsb
molecule tags Non specific mono-esterase
molecule keywords ALKALINE PHOSPHATASE
source organism Escherichia coli
missing residues 326-331, 403-410
total genus Genus: 158
ec nomenclature ec 3.1.3.1: Alkaline phosphatase.
pdb deposition date 1995-08-19
KnotProt deposition date 2014-07-31

pfam database annotations

chain Pfam Accession Code Pfam Family Identifier Pfam Description
A PF00245 Alk_phosphatase Alkaline phosphatase
Image from the rcsb pdb (www.rcsb.org)
cath code
ClassArchitectureTopologyHomologyDomain
3.40.720.10 Alpha Beta 3-Layer(aba) Sandwich Alkaline Phosphatase, subunit A Alkaline Phosphatase, subunit A 1ajaA00
3MK0A 2ANHA 3UO0A 1ALJA 1AJCA 1ED9A 3UN5A 3T02A 1ELZA 3T00A 1AUKA 1N2KA 3MK2A 3DYCA 3IGZB 1N2LA 1Y6VA 1EJJA 1ELYA 1HJKA 3M8YA 1E33P 3ED4A 3SZYA 2GA3A 1HDHA 1KHJA 1EW9A 2G9YA 1KH5A 3T01A 3TWZA 1E1ZP 1E3CP 1SHQA 1ZEBA 3SZZA 1ZEDA 1AJAA 1K7HA 1B8JA 3M7VA 3BDGB 2GLQA 1AJDA 4FDJA 1P49A 1O99A 3M8WA 3M8ZA 1EI6A 3UN3A 1KH9A 1KHKA 1KHLA 1Y7AA 3TX0A 2QZUA 1EW2A 1URAA 4FDIA 1EW8A 3DPCA 3UN2A 1ZEFA 3UNYA 1ANJA 1ALKA 1KHNA 3BDFA 1HQAA 1E2SP 1ELXA 1URBA 3BDGA 1O98A 1SHNA 1ALIA 3OT9A 3NVLA 1KH7A 1FSUA 3IGYB 1ANIA 3BDHA 3MK1A 1AJBA 1EQJA 1KH4A 1ED8A 3CMRA 2IFYA
chains in the KnotProt database with same CATH superfamily
3MK0A 2ANHA 3UO0A 1ALJA 1AJCA 1ED9A 3UN5A 3T02A 1ELZA 3T00A 1AUKA 1N2KA 3MK2A 3DYCA 3IGZB 1N2LA 1Y6VA 1EJJA 1ELYA 1HJKA 3M8YA 1E33P 3ED4A 3SZYA 2GA3A 1HDHA 1KHJA 1EW9A 2G9YA 1KH5A 3T01A 3TWZA 1E1ZP 1E3CP 1SHQA 1ZEBA 3SZZA 1ZEDA 1AJAA 1K7HA 1B8JA 3M7VA 3BDGB 2GLQA 1AJDA 4FDJA 1P49A 1O99A 3M8WA 3M8ZA 1EI6A 3UN3A 1KH9A 1KHKA 1KHLA 1Y7AA 3TX0A 2QZUA 1EW2A 1URAA 4FDIA 1EW8A 3DPCA 3UN2A 1ZEFA 3UNYA 1ANJA 1ALKA 1KHNA 3BDFA 1HQAA 1E2SP 1ELXA 1URBA 3BDGA 1O98A 1SHNA 1ALIA 3OT9A 3NVLA 1KH7A 1FSUA 3IGYB 1ANIA 3BDHA 3MK1A 1AJBA 1EQJA 1KH4A 1ED8A 3CMRA 2IFYA
chains in the KnotProt database with same CATH topology
3MK0A 2ANHA 3UO0A 1ALJA 1AJCA 1ED9A 3UN5A 3T02A 1ELZA 3T00A 1AUKA 1N2KA 3MK2A 3DYCA 3IGZB 1N2LA 1Y6VA 1EJJA 1ELYA 1HJKA 3M8YA 1E33P 3ED4A 3SZYA 2GA3A 1HDHA 1KHJA 1EW9A 2G9YA 1KH5A 3T01A 3TWZA 1E1ZP 1E3CP 1SHQA 1ZEBA 3SZZA 1ZEDA 1AJAA 1K7HA 1B8JA 3M7VA 3BDGB 2GLQA 1AJDA 4FDJA 1P49A 1O99A 3M8WA 3M8ZA 1EI6A 3UN3A 1KH9A 1KHKA 1KHLA 1Y7AA 3TX0A 2QZUA 1EW2A 1URAA 4FDIA 1EW8A 3DPCA 3UN2A 1ZEFA 3UNYA 1ANJA 1ALKA 1KHNA 3BDFA 1HQAA 1E2SP 1ELXA 1URBA 3BDGA 1O98A 1SHNA 1ALIA 3OT9A 3NVLA 1KH7A 1FSUA 3IGYB 1ANIA 3BDHA 3MK1A 1AJBA 1EQJA 1KH4A 1ED8A 3CMRA 2IFYA
chains in the KnotProt database with same CATH homology

  
#chains in the KnotProt database with same CATH superfamily
 3MK0 A;  2ANH A;  3UO0 A;  1ALJ A;  1AJC A;  1ED9 A;  3UN5 A;  3T02 A;  1ELZ A;  3T00 A;  1AUK A;  1N2K A;  3MK2 A;  3DYC A;  3IGZ B;  1N2L A;  1Y6V A;  1EJJ A;  1ELY A;  1HJK A;  3M8Y A;  1E33 P;  3ED4 A;  3SZY A;  2GA3 A;  1HDH A;  1KHJ A;  1EW9 A;  2G9Y A;  1KH5 A;  3T01 A;  3TWZ A;  1E1Z P;  1E3C P;  1SHQ A;  1ZEB A;  3SZZ A;  1ZED A;  1AJA A;  1K7H A;  1B8J A;  3M7V A;  3BDG B;  2GLQ A;  1AJD A;  4FDJ A;  1P49 A;  1O99 A;  3M8W A;  3M8Z A;  1EI6 A;  3UN3 A;  1KH9 A;  1KHK A;  1KHL A;  1Y7A A;  3TX0 A;  2QZU A;  1EW2 A;  1URA A;  4FDI A;  1EW8 A;  3DPC A;  3UN2 A;  1ZEF A;  3UNY A;  1ANJ A;  1ALK A;  1KHN A;  3BDF A;  1HQA A;  1E2S P;  1ELX A;  1URB A;  3BDG A;  1O98 A;  1SHN A;  1ALI A;  3OT9 A;  3NVL A;  1KH7 A;  1FSU A;  3IGY B;  1ANI A;  3BDH A;  3MK1 A;  1AJB A;  1EQJ A;  1KH4 A;  1ED8 A;  3CMR A;  2IFY A; 
#chains in the KnotProt database with same CATH topology
 3MK0 A;  2ANH A;  3UO0 A;  1ALJ A;  1AJC A;  1ED9 A;  3UN5 A;  3T02 A;  1ELZ A;  3T00 A;  1AUK A;  1N2K A;  3MK2 A;  3DYC A;  3IGZ B;  1N2L A;  1Y6V A;  1EJJ A;  1ELY A;  1HJK A;  3M8Y A;  1E33 P;  3ED4 A;  3SZY A;  2GA3 A;  1HDH A;  1KHJ A;  1EW9 A;  2G9Y A;  1KH5 A;  3T01 A;  3TWZ A;  1E1Z P;  1E3C P;  1SHQ A;  1ZEB A;  3SZZ A;  1ZED A;  1AJA A;  1K7H A;  1B8J A;  3M7V A;  3BDG B;  2GLQ A;  1AJD A;  4FDJ A;  1P49 A;  1O99 A;  3M8W A;  3M8Z A;  1EI6 A;  3UN3 A;  1KH9 A;  1KHK A;  1KHL A;  1Y7A A;  3TX0 A;  2QZU A;  1EW2 A;  1URA A;  4FDI A;  1EW8 A;  3DPC A;  3UN2 A;  1ZEF A;  3UNY A;  1ANJ A;  1ALK A;  1KHN A;  3BDF A;  1HQA A;  1E2S P;  1ELX A;  1URB A;  3BDG A;  1O98 A;  1SHN A;  1ALI A;  3OT9 A;  3NVL A;  1KH7 A;  1FSU A;  3IGY B;  1ANI A;  3BDH A;  3MK1 A;  1AJB A;  1EQJ A;  1KH4 A;  1ED8 A;  3CMR A;  2IFY A; 
#chains in the KnotProt database with same CATH homology
 3MK0 A;  2ANH A;  3UO0 A;  1ALJ A;  1AJC A;  1ED9 A;  3UN5 A;  3T02 A;  1ELZ A;  3T00 A;  1AUK A;  1N2K A;  3MK2 A;  3DYC A;  3IGZ B;  1N2L A;  1Y6V A;  1EJJ A;  1ELY A;  1HJK A;  3M8Y A;  1E33 P;  3ED4 A;  3SZY A;  2GA3 A;  1HDH A;  1KHJ A;  1EW9 A;  2G9Y A;  1KH5 A;  3T01 A;  3TWZ A;  1E1Z P;  1E3C P;  1SHQ A;  1ZEB A;  3SZZ A;  1ZED A;  1AJA A;  1K7H A;  1B8J A;  3M7V A;  3BDG B;  2GLQ A;  1AJD A;  4FDJ A;  1P49 A;  1O99 A;  3M8W A;  3M8Z A;  1EI6 A;  3UN3 A;  1KH9 A;  1KHK A;  1KHL A;  1Y7A A;  3TX0 A;  2QZU A;  1EW2 A;  1URA A;  4FDI A;  1EW8 A;  3DPC A;  3UN2 A;  1ZEF A;  3UNY A;  1ANJ A;  1ALK A;  1KHN A;  3BDF A;  1HQA A;  1E2S P;  1ELX A;  1URB A;  3BDG A;  1O98 A;  1SHN A;  1ALI A;  3OT9 A;  3NVL A;  1KH7 A;  1FSU A;  3IGY B;  1ANI A;  3BDH A;  3MK1 A;  1AJB A;  1EQJ A;  1KH4 A;  1ED8 A;  3CMR A;  2IFY A;
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similar chains in the KnotProt database (?% sequence similarity)
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similar chains in the pdb database (?% sequence similarity)

  
#similar chains in the KnotProt database (?% sequence similarity)
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#similar chains, but unknotted
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#similar chains in the pdb database (?% sequence similarity)
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